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  • br A typical dose dependent cytotoxic

    2020-08-18


    A typical dose dependent cytotoxic effect was apparently noticed in the SRGO treated gastric cancer cell lines (Fig. 5A,B,C). The con-centration required to destruct at least 50% of NPI-0052 is referred as IC50 value. In this study, IC50 values of each formulation were calculated in order to estimate the anticancer ability of respective formulation. The IC50 values of free SRF and SRGO were recorded as 2.57 μg/ml and 6.45 μg/ml, respectively (Fig. 5B). On the other hand, the cell viability observed with blank RGO showed the higher biocompatibility with 100% cell viability (Fig. 5A). Furthermore, it can be expected that drug–loaded nanoparticles has potent anticancer ability there by in-creasing their therapeutic efficacy. Also, SRGO treated cell group showed a high cell death with round and distorted cancer cells, whereas cells in untreated and blank RGO
    Fig. 5. Cell viability of SGC-7901 cell lines treated with (A) blank RGO (B) drug-loaded RGO and (C) Morphological imaging of SGC7901 cancer cells.
    Fig. 6. Hoechst 3382 staining of cancer cells. The cells were treated with Hoechst dyeand respective formulations for 10 min.
    treated group remained intact (Fig. 5C).
    From the Hoechst 33382 staining study (Fig. 6), it was evident that the cells in untreated groups remained intact with its round shape and intact nuclei. However, the SRF treated cells have shown a cell trans-formation with apoptosis of cancer cell lines. In particular, the SRGO treated cells were brightly stained and became smaller with irregular morphology.
    4. Conclusions
    In conclusion, we have successfully demonstrated the preparation of sorafenib coated graphene nanosheets for the treatment of gastric cancers. SRGO were obtained with a transparent and smooth mor-phology. The drug loaded SRGO has presented significant cytotoxic effect against SGC7901 cancer cells when compared to that of the free SRF and blank NPs in the equivalent concentrations. Additionally, from the Hoechst 33382 staining study it was evident that the cells in un-treated groups remained intact with its round shape and intact nuclei while the SRGO treated cells have shown a cell transformation with apoptosis of gastric cancer cell lines. Based on these results, we can conclude that SRGO might extend an enormous prospective in the treatment of gastric cancers.
    Acknowledgements
    Authors are thankful to Tangshan Gongren Hospital for supporting to do this research.
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    Journal of Photochemistry & Photobiology, B: Biology
    journal homepage: www.elsevier.com/locate/jphotobiol
    Biosynthesized of reduced graphene oxide nanosheets and its loading with T NPI-0052 paclitaxel for their anti cancer effect for treatment of lung cancer